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A cDNA encoding a
putative SPF1-type DNA-binding protein from
cucumber.
Kim DJ, Smith SM, Leaver
CJ.
Department of Plant Sciences, University of Oxford,
UK.
A cDNA clone encoding a polypeptide with homology to the
novel SPF1 DNA-binding protein of sweet potato has been isolated from a
cDNA library from RNA of senescing cucumber (Cucumis sativus, L.)
cotyledons. Comparison of the two sequences reveals similar features
which may be important in the evolution and function of this protein,
including a duplicated region of about 56 amino acids (aa). The first
half of the duplicated region is enriched in basic aa and is very highly
conserved, both within and between each polypeptide. In contrast, the
second half of the duplicated region is poorly conserved within each
polypeptide, but highly conserved when cucumber and sweet potato
sequences are compared. Southern blot analysis with cucumber DNA shows a
simple hybridisation pattern indicating one or very few genes. Northern
blot analysis shows that the expression of the cucumber gene increases
in cotyledons as they expand and become photosynthetic and remains high
in senescence. The possibility that the cucumber SPF1-type protein may
be involved in carbohydrate regulation of gene expression is
discussed.
PMID: 9055825 [PubMed - indexed for MEDLINE]
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